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Registros recuperados: 13
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Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles BJMBR
Sá,N.A.R.; Bruno,J.B.; Guerreiro,D.D.; Cadenas,J.; Alves,B.G.; Cibin,F.W.S.; Leal-Cardoso,J.H.; Gastal,E.L.; Figueiredo,J.R..
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concentrations on the in vitro culture of caprine preantral follicles enclosed in ovarian tissue. Randomized ovarian fragments were fixed immediately (non-cultured treatment) or distributed into five treatments: α-MEM+ (cultured control), α-MEM+ supplemented with ascorbic acid at 50 μg/mL (AA), and anethole at 30 (AN30), 300 (AN300), or 2000 µg/mL (AN2000), for 1 or 7 days. After 7 days of culture, a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Anethole; ROS; Primordial follicle; Caprine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800602
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Artrite-encefalite caprina (CAE): diagnóstico aboratorial. Infoteca-e
VESCHI, J. L. A.; CASTRO, R. S. de; NASCIMENTO, S. A. do; RAMOS, E. M.; ZAFALON, L. F..
bitstream/item/81175/1/INT105.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Artrite-encefalite caprina; Disease.; Caprine; Caprino; Doença.
Ano: 2012 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/955497
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Avaliação da biodiversidade da Caatinga para controle de nematódeos gastrintestinais de caprinos e ovinos. Infoteca-e
NOGUEIRA, D. M..
Na produção animal, a verminose é um dos fatores limitantes da caprinocultura, sendo considerada a principal doença responsável pelas elevadas perdas econômicas no rebanho. Associado a esse problema está o uso indiscriminado de drogas anti--helmínticas alopáticas que é responsável pela seleção de nematódeos resistentes ou de nematódeos que desenvolvem resistência aos anti-helmínticos. Esta atividade teve como objetivos testar diferentes dosagens em caprinos e/ou ovinos de plantas medicinais já citadas na literatura como detentoras de ação anti-helmíntica e avaliar a eficácia das plantas medicinais da Caatinga conhecidas popularmente como anti-helmínticas, porém ainda não citadas na literatura. Foram utilizados caprinos e ovinos mestiços para cada...
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Caprine; Ovine; Caprino; Ovino; Produção animal; Biodiversidade; Caatinga; Doença; Verminose.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/937157
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Clinical features of caprine arthritis-encephalitis infections Arq. Bras. Med. Vet. Zootec.
Lara,M.C.C.S.H; Birgel Junior,E.H.; Gregory,L.; Birgel,E.H..
In a clinical survey of caprine arthritis-encephalitis in 14 naturally infected herds in the State of São Paulo, Brazil, 17.1% (64/374) of seroreagents presented the articular form of the disease and 6.6% (17/249) of the seroreagent females presented the mammary form of the disease.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine; Arthritis- encephalitis; Mastitis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600005
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Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured Arq. Bras. Med. Vet. Zootec.
Lima-Verde,I.B.; Matos,M.H.T.; Bruno,J.B.; Martins,F.S.; Santos,R.R.; Báo,S.N.; Luque,M.C.A.; Vieira,G.A.B.; Silveira,E.R.; Rodrigues,A.P.R.; Figueiredo,J.R.; Oliveira,M.A.L.; Lima,P.F..
The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Caprine; Preantral follicle; Α-tocopherol; Ternatin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009
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Elephant grass ensiled with wheat bran compared with corn silage in diets for lactating goats R. Bras. Zootec.
Silva,Jacianelly Karla da; Oliveira,Juliana Silva de; Medeiros,Ariosvalo Nunes de; Santos,Edson Mauro; Magalhães,Tamires da Silva; Ramos,Alenice Ozino; Bezerra,Higor Fábio Carvalho.
The objective of this study was to evaluate the use of wheat bran as an additive in elephant-grass silage on intake and digestibility of the nutrients, ingestive behavior, and yield and chemical composition of milk. Eight goats with 45 days of lactation were distributed in a (4 × 4) Latin square design.The treatments consisted of corn silage (CS), elephant-grass silage without wheat bran (EGS), elephant-grass silage with 10% wheat bran (EGS+10%WB), and elephant-grass silage with 20% wheat bran (EGS+20% WB). There was no difference in dry matter (DM) intake between diets EGS and CS in g d−1. However, the animals fed EGS+10%WB had lower DM and organic matter (OM) intakes than the animals fed CS in g kg−1 d−1 of body weight. There were lower non-fiber...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additive; Caprine; Ethology; Milk; Milk composition; Nutritive value.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001100618
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Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro Ciência Rural
Leitão,Cintia Camurça Fernandes; Costa,José Jackson Nascimento; Saraiva,Márcia Viviane Alves; Araújo,Valdevane Rocha; Figueiredo,José Ricardo; Hurk,Robert van den; Silva,José Roberto Viana.
This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pre-antral follicles; Caprine; Culture; Oocyte; Antrum formation; FSH-R.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100018
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Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil BJM
Oliveira,Rosângela C.; Yamaguti,Maurício; Marques,Lucas M.; Buzinhani,Melissa; Rizzo,Huber; Meira-Junior,Enoch Brandão de Souza; Guimarães,Ana Márcia S.; Gregory,Lilian; Timenetsky,Jorge.
Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ureaplasma; Ovine; Caprine; Molecular characterization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560
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Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles BJMBR
Cunha,E.V.; Costa,J.J.N.; Rossi,R.O.D.S.; Silva,A.W.B.; Passos,J.R.S.; Portela,A.M.L.R.; Pereira,D.C.S.T.; Donato,M.A.M.; Campello,C.C.; Saraiva,M.V.A.; Peixoto,C.A.; Silva,J.R.V.; Santos,R.P..
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine; Antrum formation; FSH-R; PCNA; Ultrastructure.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300245
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Possibility of vertical transmission of caprine arthritis-encephalitis virus in neonate kids Arq. Bras. Med. Vet. Zootec.
Lara,M.C.C.S.H.; Birgel Junior,E.H.; Birgel,E.H..
Estudou-se a capacidade do vírus da artrite-encefalite dos caprinos (CAEV) infectar o feto ou o cabrito neonato pela via de transmissão transplacentária ou no momento do parto. Foram utilizados 26 cabritos recém-nascidos, filhos de cabras sororreagentes aos antígenos do CAEV e que nasceram de partos eutócicos. Na pesquisa de anticorpos séricos anti-CAEV, foi utilizada a técnica de imunodifusão em gel de ágar. Nenhum cabrito nasceu sororreagente aos antígenos do vírus, indicando que a possibilidade de transmissão vertical transplacentária da infecção foi menor do que 3,8% (< 1:26).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine; Caprine arthritis-encephalitis; Virus; CAEV; Lentivirus; Transmission.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400019
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Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen Arq. Bras. Med. Vet. Zootec.
Aguiar,G.V.; van Tilburg,M.F.; Catunda,A.G.V.; Celes,C.K.S.; Lima,I.C.S.; Campos,A.C.N.; Moura,A.A.A.; Araújo,A.A..
The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity) were analyzed. It was observed a reduction (P<0.05) in normal sperm morphology, fructose, citric acid, P, Mg and total protein concentration during the dry season, which did not affect the motility, vigor, volume and sperm concentration. Phospholipase A2 activity was increased during the dry season (P<0.05). The analysis of the semen cooled at 4ºC during 48 hours showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Semen; Spermatozoa; Season; Cooling; Caprine.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100002
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Thoracic aortic aneurysm in a buck associated with caseous lymphadenitis Arq. Bras. Med. Vet. Zootec.
Pinheiro,R.R.; Eloy,A.M.X.; Alves,F.S.F.; Andrioli,A.; Santiago,L.B..
This paper reports the clinical, bacteriological and pathological findings of a thoracic aortic aneurysm in a four-year-old Anglo-Nubian goat buck, related to a framework of visceral caseous lymphadenitis. General clinical examination showed heart rate of 75 beats per minute, respiratory rate of 20 movements per minute and ruminal movements of four movements per minute. Superficial lymph nodes were normal upon palpation. Rectal temperature was slightly high (40.5°C). Blood test showed an intense leukocytosis (54,000/µL), characterized by strong neutrophil shift to the left. At necropsy, a large blood clot was detected in the thoracic cavity. The thickening of the myocardium and dilatation of the aorta in the thoracic portion, presenting a saculiform format...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abscesses; Caprine; Corynebacterium pseudotuberculosis; Leukocytosis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300012
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Validation of a PCR Assay for Chlamydophila abortus rRNA gene detection in a murine model BABT
Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Negrão,Fábio Juliano; Lunardi,Michele; Freitas,Julio Cesar de.
Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens and organ fragments, such as placenta, fetal organs, vaginal secretions, and semen. The aim of this study was to develop a PCR assay for the amplification of an 856-bp fragment of the rRNA gene of the Chlamydiaceae family. The PCR assay was evaluated using organs from 15 mice experimentally infected with the S26/3 reference strain of C. abortus. The results of the rRNA PCR were compared to the results from another PCR system (Omp2 PCR) that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Ovine; Caprine; Reproductive failures; Chlamydophila abortus; PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700014
Registros recuperados: 13
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